Easy translator 8.2 serial number4/17/2023 The spectral diversity of the ChromaTide UTP, ChromaTide dUTP and ChromaTide OBEA-dCTP nucleotides ( Characteristics of Molecular Probes ChromaTide and aha labeled nucleotides-Table 8.5) gives researchers significant flexibility in choosing a label that is compatible with a particular optical detection system or multicolor experiment. The Alexa Fluor 546 and Alexa Fluor 647 OBEA-dCTP conjugates ( C21555, C21559) also have a built-in spacer that reduces possible steric interference caused by the presence of the dye. The ChromaTide OBEA-deoxycytidine triphosphates (OBEA-dCTP) are modified at the N-4 position of cytosine using a 2-aminoethoxyethyl (OBEA) linker ( Figure 8.2.3). Longer spacers typically result in brighter conjugates and increased hapten accessibility for secondary detection reagents. The number in the product's name (e.g., the "12" in ChromaTide fluorescein-12-dUTP) indicates the net length of the spacer in atoms. In addition to this four-atom bridge, several of these nucleotides contain a seven- to 10-atom spacer that further separates the dye from the base. The aminoalkynyl linker between the fluorophore and the nucleotide in the ChromaTide UTP and dUTP nucleotides is designed to reduce the fluorophore's interaction with enzymes or target binding sites. The C-5 position of UTP and dUTP is not involved in Watson–Crick base-pairing and so interferes little with probe hybridization. The ChromaTide UTP and dUTP nucleotides are modified at the C-5 position of UTP or dUTP via a unique aminoalkynyl linker ( Figure 8.2.2).
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